Technologist II

Position Summary:

Technologist II (Molecular) will need to have a broad skill set and good troubleshooting skills within the laboratory setting. Be able to work with scientists to develop and perform assays for molecular biology projects through assay development, validation, and execution phases in a timely manner. Perform assays according to SOPs and support development of new procedures and improvement of existing SOPs. Also, familiar and operating under CLIA. Responsible for training other staff.

Essential Functions:

• Skilled with molecular biology techniques
• Ability and willingness to learn and execute techniques related to RNA and DNA extractions, qPCR, NGS or other molecular biology platforms
• Run quality control samples; Run clinical samples and generate data
• Maintain a stock of all needed reagents and consumables
• Perform daily, weekly, monthly instrument maintenance and trouble-shooting as required
• Flexibility in shift work
• Work with scientists to carry out qualification of assays for new projects
• Work with QA (Quality Assurance) to perform good documentation, and to ensure the lab operating under CLIA, GLP and GCP standards
• Train new personnel on project related assays, daily lab work and SOPs.
• Participate in cross-functional process improvement activities
• May need to complete freezer transfers, generate reports, and coordinate with QC and entry of locations into the BSI
• Other duties as assigned

Qualifications:

Minimum Required:

• Bachelor's Degree (Life Sciences, Biology or other related discipline)
• More than 2 years of prior related laboratory experience

Other Required:

• Position requires occasional long hours, work on the evenings, weekends, and holidays.
• Someone who is a good team player, detail-oriented and organized, can work under pressure and has good oral and written communication skills
• This person can contribute to quality control of reagents through a variety of tests, and writing of SOPs
• Superior attention to detail, ability and willingness to effectively scrutinize data and ability to work well in a group environment
• Must possess strong computational skills, preferably experienced with Word, Excel, Power Point, etc.
• Excellent use of judgment and discretion required
• Ability to lift and carry between 1-15 lbs.; Ability to lift up to 50 lbs. overhead with assistance; frequently required to sit, grasp, and use right/left finger dexterity; May be exposed to a cold freezer environment (Freezer temperatures range between -20ºC -196ºC)
• Must be able to read, write, speak fluently and comprehend the English language

Preferred:

• 4 years of related experience, preferably in NGS core facility or NGS lab.
• MB (ASCP) certification a plus but not a requirement
• Wet lab experience in the entire NGS workflow (Illumina or Ion Torrent), from library preparation to sequencing.
• Experience in quantitative PCR (qPCR), digital droplet PCR (ddPCR) and NanoString is a plus.
• Experience in DNA and RNA extractions from a variety of sources is a plus.
• Solid lab experience with multiple skill sets
• A solid understanding of current GLP or CAP/CLIA/ISO standards
• Have a documentation background in a laboratory setting with respect to regulatory guidelines (cGMP, cGLP or ISO) and SOPs